The recently-formed Gloucester Railway Carriage and Wagon Museum has appealed for help to save the Gloucester Horton Road Motive Power Depot from demolition.
Known as 85B, the brick-built shed is under threat from being demolished with the site used for housing.
After being formed in 2016 by a group of local enthusiasts, the museum has held several meetings with site owner Network Rail with a view to buying it.
At first, Network Rail seemed happy to sell if the museum could raise the purchase price and get planning permission.
However, Network Rail has since said that the site is only available for residential redevelopment.
The associated Gloucester Railway Carriage & Wagon Company has drawn up plans for the manufacture and restoration of carriages and wagons for heritage railways. It also hopes to work with main line tour operators to offer a servicing point for their locomotives.
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